- Excitor filter: allows only blue light to illuminate the retina. It reduces the amount of non-fluorescent light that can reach the film, and allows for maximum excitation of the fluorescein.
- Barrier filter: allows only yellow-green light (from the fluorescence) to reach the camera. It absorbs the blue light used to illuminate the retina.
- Camera with black and white film.
Technique
- Baseline color and red-free filtered images are taken prior to injection. This allows for the recognition of autofluorescence of the retinal tissues.
- Injection of approximately 5cc of sodium fluorescein into a vein in the arm or hand.
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A series of black-and-white photographs are taken of the retina after the fluorescein reaches the retinal circulation (approximately 10 seconds after injection). Photos are taken approximately once every second for about 20 seconds, then less often. A delayed image is obtained at 5 minutes.
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A filter is placed in the camera so only the fluorescent, yellow-green light (530 nm) is recorded. The camera may however pick up signals from pseudofluorescence or autofluorescence. In pseudofluorescence, non-fluorescent light is imaged. This occurs when blue light reflected from the retina passes through the filter, a problem with older filters. In autofluorescence, fluorescence from the eye occurs without injection of the dye. This may be seen with optic nerve head drusen, astrocytic hamartoma, or calcific scarring.
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Black-and-white photos give better contrast than color photos, which aren't necessary since only one color is being transmitted though the filter
Normal Circulatory filling
- 0 seconds — injection of fluorescein
- 9.5 sec — posterior ciliary arteries
- 10 sec — choroidal flush (or "pre-arterial phase")
- 10-12 sec — retinal arterial stage
- 13 sec — capillary transition stage
- 14-15 sec — early venous stage (or "lamellar stage", "arterial-venous stage")
- 16-17 sec — venous stage
- 18-20 sec — late venous stage
- 5 minutes — late staining
Fluorescein enters the ocular circulation from the internal carotid artery via the ophthalmic artery. The ophthalmic artery supplies the choroid via the short posterior ciliary arteries and the retina via the central retinal artery, however, the route to the choroid is typically less circuitous than the route to the retina. This accounts for the short delay between the "choroidal flush" and retinal filling.
Pathologic Findings
Causes of hyperfluorescence:
- leaking (i.e. capillary leakage, aneurysm, neovascularization)
- pooling (serous retinal detachment)
- staining
- transmission defects
- abnormal vessels
Causes of hypofluorescence:
- optical barrier (i.e. blood)
- filling defect (capillary blockage)
Among the common groups of ophthalmologic disease, fluorescein angiography can detect diabetic retinopathy, vein occlusions, retinal artery occlusions, edema of the optic disc, and tumors.
Other types of fluorescent angiography
- Isocyanide green angiography
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